AlleInformasjon om flåttenBorreliose: UtbredelseBorreliose: Diagnostikk og behandlingTBE: UtbredelseTBE: Diagnostikk og behandling
Tilbake

Prevalence and genotypes of Borrelia burgdorferi sensu lato infection in Ixodes ricinus ticks in Southern Norway

TittelPrevalence and genotypes of Borrelia burgdorferi sensu lato infection in Ixodes ricinus ticks in Southern Norway
ForfatterKjelland V, Stuen S, Skarpaas T, Slettan A.
Årstall2010
EmnerBorrelia
KommentarI gjennomsnitt er omtrent 25 % av flåtten på Sørlandet infisert av Borrelia-bakterien, men det finnes store variasjoner både fra sted til sted og på ulike tidspunkt. Alle de kjente Borrelia-artene som forårsaker sykdom, B. afzelii, B. garinii, B. burgdorferi sensu stricto og B. valaisiana, ble påvist.
SammendragFrom April to October 2007, host-seeking Ixodes ricinus ticks were collected from four locations in Southern Norway; Farsund, Mandal, Søgne and Tromøy, respectively. 210 larva, 1130 nymphs and 449 adults were investigated for infection with Borrelia burgdorferi sensu lato (s.l.) by real-time polymerase chain reaction (PCR) amplification of the 16S rRNA gene. The total percentage of B. burgdorferi s.l. in nymphal and adult ticks was determined as 31.3% in Farsund, 25.2% in Mandal, 22.3% in Søgne and 22.1% in Tromøy, respectively. Larvae were pooled in groups of 10 before analysis, and Borrelia infection was detected in one of 21 pools of larvae. B. burgdorferi s.l. were genotyped by melting curve analysis after real-time PCR amplification of the hbb gene, or by direct sequencing of the PCR amplicon generated from the rrs (16S)-rrl (23S) intergenetic spacer. The most prevalent B. burgdorferi genospecies identified were B. afzelii (61.6%), followed by B. garinii (23.4%) and B. burgdorferi sensu stricto (10.6%). B. valaisiana (4.5%) was identified in Norwegian ticks for the first time. Mixed infections were observed in 0.3% of the infected ticks. A higher prevalence of B. burdorferi s.l. was found in present study than what has been shown in previous Nordic studies.
Lenkehttp://www.ncbi.nlm.nih.gov/pubmed/20429719